Skip to main content
Figure 1 | Journal of Inflammation

Figure 1

From: Signaling pathways involved in LPS induced TNFalpha production in human adipocytes

Figure 1

Activation of p38 MAP Kinase and NFkappaB pathways regulate LPS induced TNFalpha synthesis from human mature adipocytes. Panel A: Effect of NFkappaB and p38 inhibitors on TNFalpha secretion. The concentrations of TNFalpha (pg/mL) were determined at 6 hours in the medium of mature adipocyte cultures treated or not with: 1 μg/mL of LPS alone; LPS and increasing concentrations of NFkappaB inhibitor (NFkBi 0.1, 0.5 and 1 μM); LPS and increasing concentrations of p38 MAP kinase inhibitor (SB, 0.1, 0.5, 1 and 5 μM); LPS and NFkBi (1 μM) + SB (1 μM). The graph represents the mean ± SE of the results from one patient (n = 6 for each condition), representative of three experiments on three different patients. ***P < 0.001%, *P < 0.05%, versus cells treated with LPS alone. Panel B: TNFalpha gene expression. TNFalpha gene expression was determined at 4, 5 and 6 hours of treatment in mature adipocyte cultures, treated or not with: 1 μg/mL of LPS alone; LPS and NFkappaB inhibitor (NFkBi, 1 μM); LPS and p38 MAP kinase inhibitor (SB, 1 μM); LPS and NFkBi (1 μM) + SB (1 μM). The graph represents the mean ± SD of the results from one patient (n = 5 for each condition), representative of two experiments on two different patients. ***P < 0.001%, *P < 0.05%, versus cells treated with LPS alone.

Back to article page