Effect of immunoneutralization of TLR4 and TLR2 with their respective antibodies and siRNA mediated suppression of TLR2 and TLR4 on IL6 expression. Cells were treated with 5 μg/ml neutralizing antibodies against TLR2 (TLR2ab) and TLR4 (TLR4ab) for 1 hour before treatment with LPS (100 ng/ml) and PEP (10 μg/ml) for 6 hours (3A). Both antibodies inhibit (P < 0.05) the induction of IL6 mRNA by their respective receptors. In cells treated with TLR2 and TLR4 specific siRNAs expression of TLR2 and TLR4 was reduced by their respective siRNAs (3B). Induction of IL6 expression by treatment with peptidoglycan was attenuated in cells treated with TLR2 siRNA, but not the negative siRNA (vesirna) or TLR4 siRNA (3C). Bars represent means and ± SEM of this normalized expression from 4 different replicates. Bars with different superscripts are different (P < 0.05).