Figure 5

Pharmacodynamic effects of MK0812 in vivo. BALB/c mice were administered the indicated dosage of vehicle (MC) or MK0812 by oral gavage. 2 h later, peripheral blood CD11b⁺Ly6C^hi monocyte numbers were determined as in Figure 2 (A) and plasma concentrations of CCL2 (B) and CCL7 (C) were determined. ***p < 0.001 and **p < 0.01 compared to MC group. D, The effect of MK0812 (3 mg/kg, p.o.) or vehicle (MC) on CCL2 and CCL3 mediated changes in CD11b⁺Ly6C^hi monocyte numbers were evaluated as in Figure 4. **p < 0.01 compared to PBS group. E, BALB/c mice were dosed with vehicle (MC) or MK0812 (30 mg/kg, p.o.) and 2 h later, blood was collected and analyzed for white blood cell counts using an ADVIA system to quantify total leukocytes, lymphocytes, monocytes, neutrophils, basophils and eosinophils. *p < 0.05 compared to MC group. The data presented are the mean ± S.E.M. of six mice per group and are representative of three independent experiments.