Figure 4

MK0812 inhibits CCL2-mediated monocyte migration in vivo. A, BALB/c mice were administered the indicated dosage of vehicle (MC) or MK0812 by oral gavage. 1 h later, the mice were injected with PBS or 300 ng CCL2 i.v. 30 min later, peripheral blood CD11b⁺Ly6C^hi monocyte numbers were determined as in Figure 2. B, The plasma concentrations of MK0812 were determined from the animals used in (A) and plotted versus the dosage of MK0812 administered. The IC₅₀ for inhibition of CCL-2 mediated chemotaxis (see Figure 3) is indicated by a dashed line. The data presented are the mean ± S.E.M. of six mice per group and are representative of three independent experiments. **p < 0.01 compared to MC/CCL2 group.