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Table 3 Total RNAs from untreated and LPS-treated endothelial cells were isolated and reverse transcribed.

From: Upregulation of prolylcarboxypeptidase (PRCP) in lipopolysaccharide (LPS) treated endothelium promotes inflammation

Biomarker

(mRNA)

RT-PCR products Intensity (arbitrary Unit)

 

HUVEC

LPS-HUVEC

BKB1R

0

57

BKB2R

320

234

AT1

127

258

AT2

340

560

tPA

243

340

PAI-1

402

905

VWF

297

844

ICAM-1

0

97

eNOS

176

105

  1. PCR products were resolved in 1% agarose gel and detected by ethidium bromide. The intensity of PCR products were quantified after normalizing to GAPDH. Bradykinin B1 receptor; BKB1R, Bradykinin B2 receptor; BKB2R, angiotensin type 1 receptor; AT1, angiotensin type 2 receptor; AT2, tissue plasminogen activator; tPA, plasminogen activator inhibitor 1; PAI-1, von Willebrand factor; VWF, intercellular adhesion molecule-1; ICAM, endothelial nitric oxide synthase; eNOS.