Table 1 Primer pairs used to analyze the expression of nine biomarkers of coagulation activation, fibrinolysis, endothelial injury, and inflammation.
Sense primer | Position On cDNA | Antisense primer | Position on cDNA | PCR Product Length (bp) | |
|---|---|---|---|---|---|
PRCP | 5'-ATGGGCCGCCGAGCCCTCCTG-3' | 114–134 | 5'-GGTTGGTTGGCAAGTGTAGG-3' | 225-206 | 111 |
BKB1R | 5'-CACTTTGCAAGGATGGTGGAGTTG-3' | 764–787 | 5'-GGAGGCCAGGATGTGATAGTTGAA-3' | 853-830 | 89 |
BKB2R | 5'-CTGGGTGTTTGGAGAGGTGT-3' | 479–499 | 5'-ACGAGCATCAGGAAGCAGAT-3' | 565-545 | 86 |
tPA | 5'-GGCTGTGGACAGAAGGATGT-3' | 1676–1695 | 5'-TGCACTCTTCCCTCTCCTGT-3' | 1909-1890 | 233 |
PAI-1 | 5'-CAGACCAAGAGCCTCTCCAC-3' | 1073–1092 | 5'-GACTGTTCCTGTGGGGTTGT-3' | 1254-1235 | 181 |
ICAM-1 | 5'-GGCTGGAGCTGTTTGAGAAC-3' | 644–663 | 5'-ACTGTGGGGTTCAACCTCTG-3' | 845-826 | 201 |
vWF | 5'-TGCTGACACCAGAAAAGTGC-3' | 3343–3362 | 5'-AGTCCCCAATGGACTCACAG-3' | 3539-3520 | 196 |
eNOS | 5'-AGCATCCCTACTCCCACCA-3' | 419–437 | 5-'ACCTCCCAGTTCTTCACACG-3' | 520-501 | 101 |
GAPDH | 5'-GAGTCAACGGATTTGGTCGT-3' | 122–141 | 5'-GACAAGCTTCCCGTTCTCAG-3' | 306-287 | 184 |