Figure 1

LPS enhances prekallikrein activation and PRCP expression in HUVEC. Panel A: total RNA was isolated from HUVEC and LPS treated HUVEC (LPS-HUVEC) cells and then amplified by RT-PCR. Amplified DNA (100 bp fragment) was resolved on a 1.5% agarose gel. Panel B: 20 nM PK in the absence or presence of inhibitor was incubated with 20 nM HK bound to untreated, LPS-pretreated, or PRCP-siRNA transfected cells pretreated with LPS at 37°C. The liberation of paranitroanilide (pNA) from substrate (S2302) by kallikrein was measured at 405 nm. *p < 0.01 vs. untreated cells. The presented are the mean ± SEM of triplicate points of 10 independent experiments.