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Table 3 Effects of GF10903X on the Mn2+ quenching of fura-2 fluorescence in PAF-activated neutrophils, as well as the effects of GF10903X on 45Ca2+ uptake by PAF-stimulated cells.

From: Protein kinase C promotes restoration of calcium homeostasis to platelet activating factor-stimulated human neutrophils by inhibition of phospholipase C

System Magnitude of the decrement in fura-2 fluorescence intensity in the presence of Mn2+ (cm): 45Ca2+ Uptake(pmol 45Ca2+/107 cells)
  1 min 3 min 5 min 3 min 5 min
PAF (20 nM)
Control
1.8 ± 0.13 3.4 ± 0.2 4.2 ± 0.3 46 ± 6 53 ± 4
GF10903X
(0.5 μM)
2.2 ± 0.13* 5.3 ± 0.3* 6.5 ± 0.2*   
GF10903X
(1 μM)
2.1 ± 0.1* 6.0 ± 0.23* 7.3 ± 0.3* 125 ± 18* 116 ± 16*
PAF (200 nM)
Control
2.6 ± 0.16 6.3 ± 0.6 7.4 ± 0.7 48 ± 12 112 ± 5
GF10903X
(0.5 μM)
2.9 ± 0.35 6.9 ± 0.6 8.3 ± 0.6   
GF10903X
(1 μM)
2.5 ± 0.26 6.5 ± 0.7 8.2 ± 0.7 41 ± 2 105 ± 4
  1. The results of 5 - 8 experiments are expressed as the mean percentage magnitude of decrement (Mn2+ quenching) or mean percentage of control (45Ca2+ uptake) ± S.E.M. * P < 0.05 for comparison with the untreated control system.