Protein kinase C promotes restoration of calcium homeostasis to platelet activating factor-stimulated human neutrophils by inhibition of phospholipase C

Table 3 Effects of GF10903X on the Mn²⁺ quenching of fura-2 fluorescence in PAF-activated neutrophils, as well as the effects of GF10903X on ⁴⁵Ca²⁺ uptake by PAF-stimulated cells.

System	Magnitude of the decrement in fura-2 fluorescence intensity in the presence of Mn²⁺ (cm):			⁴⁵Ca²⁺ Uptake(pmol ⁴⁵Ca²⁺/10⁷ cells)
	1 min	3 min	5 min	3 min	5 min
PAF (20 nM) Control	1.8 ± 0.13	3.4 ± 0.2	4.2 ± 0.3	46 ± 6	53 ± 4
GF10903X (0.5 μM)	2.2 ± 0.13*	5.3 ± 0.3*	6.5 ± 0.2*
GF10903X (1 μM)	2.1 ± 0.1*	6.0 ± 0.23*	7.3 ± 0.3*	125 ± 18*	116 ± 16*
PAF (200 nM) Control	2.6 ± 0.16	6.3 ± 0.6	7.4 ± 0.7	48 ± 12	112 ± 5
GF10903X (0.5 μM)	2.9 ± 0.35	6.9 ± 0.6	8.3 ± 0.6
GF10903X (1 μM)	2.5 ± 0.26	6.5 ± 0.7	8.2 ± 0.7	41 ± 2	105 ± 4

The results of 5 - 8 experiments are expressed as the mean percentage magnitude of decrement (Mn²⁺ quenching) or mean percentage of control (⁴⁵Ca²⁺ uptake) ± S.E.M. * P < 0.05 for comparison with the untreated control system.

ISSN: 1476-9255