Table 2 Effects of staurosporine and GF10903X, in the presence or absence of EGTA, on cytosolic Ca2+ concentrations of PAF-activated neutrophils, as well as the effects of U73122 added 10 - 15 sec after PAF on cytosolic Ca2+ concentrations.
System | Peak (nM) | Plateau (min) | Magnitude of decrement (nM) from peak measured at: | |||
|---|---|---|---|---|---|---|
1 min | 2 min | 3 min | 5 min | |||
PAF (200 nM) Control (n = 12) | 270 ± 8 | 0.8 ± 0.06 | 80 ± 5 | 116 ± 6 | 168 ± 8 | |
Staurosporine (400 nM) (n = 6) | 290 ± 16 | 1.03 ± 0.08 | 60 ± 11* | 92 ± 18 | 136 ± 20 | |
GF10903X (0.5 μM) (n = 9) | 274 ± 9 | 1.11 ± 0.05* | 52 ± 5* | 72 ± 6* | 112 ± 7* | |
GF10903X (1 μM) (n = 9) | 270 ± 8 | 1.2 ± 0.07* | 44 ± 6* | 64 ± 8* | 100 ± 8* | |
EGTA (n = 10) | 246 ± 4 | 100 ± 5 | 164 ± 6 | |||
EGTA + Staurosporine (n = 7) | 246 ± 8 | 76 ± 5+ | 136 ± 9+ | |||
EGTA + GF10903X (0.5 μM) (n = 7) | 238 ± 10 | 78 ± 5+ | 132 ± 6+ | |||
EGTA + GF10903X (1 μM) (n = 7) | 246 ± 9 | 76 ± 3+ | 132 ± 6+ | |||
U73122 (2 μM) (n = 4) | 266 ± 11 | 0.4 ± 0.05 | 120 ± 9 | 140 ± 7 | ||
U73122 + Staurosporine (n = 3) | 290 ± 12 | 0.43 ± 0.06 | 112 ± 15 | 132 ± 9 | ||
U73122 + GF10903X (0.5 μM) (n = 3) | 278 ± 11 | 0.43 ± 0.07 | 92 ± 16 | 116 ± 9 | ||
U73122 + GF10903X (1 μM) (n = 3) | 262 ± 8 | 0.52 ± 0.05** | 92 ± 11 | 108 ± 8** | ||