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Table 2 Effects of staurosporine and GF10903X, in the presence or absence of EGTA, on cytosolic Ca2+ concentrations of PAF-activated neutrophils, as well as the effects of U73122 added 10 - 15 sec after PAF on cytosolic Ca2+ concentrations.

From: Protein kinase C promotes restoration of calcium homeostasis to platelet activating factor-stimulated human neutrophils by inhibition of phospholipase C

System Peak
(nM)
Plateau
(min)
Magnitude of decrement (nM) from peak measured at:
    1 min 2 min 3 min 5 min
PAF (200 nM)
Control (n = 12)
270 ± 8 0.8 ± 0.06   80 ± 5 116 ± 6 168 ± 8
Staurosporine
(400 nM) (n = 6)
290 ± 16 1.03 ± 0.08   60 ± 11* 92 ± 18 136 ± 20
GF10903X (0.5 μM)
(n = 9)
274 ± 9 1.11 ± 0.05*   52 ± 5* 72 ± 6* 112 ± 7*
GF10903X (1 μM)
(n = 9)
270 ± 8 1.2 ± 0.07*   44 ± 6* 64 ± 8* 100 ± 8*
EGTA
(n = 10)
246 ± 4   100 ± 5 164 ± 6   
EGTA + Staurosporine
(n = 7)
246 ± 8   76 ± 5+ 136 ± 9+   
EGTA + GF10903X (0.5 μM)
(n = 7)
238 ± 10   78 ± 5+ 132 ± 6+   
EGTA + GF10903X (1 μM)
(n = 7)
246 ± 9   76 ± 3+ 132 ± 6+   
U73122 (2 μM)
(n = 4)
266 ± 11 0.4 ± 0.05 120 ± 9 140 ± 7   
U73122 + Staurosporine
(n = 3)
290 ± 12 0.43 ± 0.06 112 ± 15 132 ± 9   
U73122 + GF10903X (0.5 μM)
(n = 3)
278 ± 11 0.43 ± 0.07 92 ± 16 116 ± 9   
U73122 + GF10903X (1 μM)
(n = 3)
262 ± 8 0.52 ± 0.05** 92 ± 11 108 ± 8**   
  1. The results are expressed as the mean percentage of control ± S.E.M. * P < 0.05 for comparison with the untreated control system and +P < 0.05 or ** P < 0.05 for comparison with EGTA or U73122-treated neutrophils, respectively. Basal Ca2+ concentrations were 80 ± 8 nM.