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Table 1 Effects of staurosporine and GF10903X, in the presence or absence of rolipram, on cytosolic Ca2+ concentrations of PAF- activated neutrophils, as well as the effects of GF10903X on cytosolic Ca2+ concentrations in FMLP-stimulated cells.

From: Protein kinase C promotes restoration of calcium homeostasis to platelet activating factor-stimulated human neutrophils by inhibition of phospholipase C

System Peak
(nM)
Plateau
(min)
Magnitude of decrement (nM) from peak measured at:
    1 min 2 min
PAF (20 nM) Control
(n = 6)
278 ± 16 0.4 ± 0.05 108 ± 10 168 ± 12
Staurosporine (400 nM)
(n = 4)
290 ± 8 1.0 ± 0.05* 52 ± 4* 160 ± 15
GF10903X (0.5 μM)
(n = 7)
258 ± 4 1.0 ± 0.04* 32 ± 2* 124 ± 5*
GF10903X (1 μM)
(n = 7)
266 ± 10 1.1 ± 0.02* 24 ± 3* 104 ± 4*
Rolipram (2 μM)
(n = 4)
274 ± 4 0.13 ± 0.03 188 ± 4 216 ± 4
Staurosporine + Rolipram
(n = 4)
286 ± 6 0.2 ± 0.06 144 ± 8** 200 ± 8
GF10903X (0.5 μM) + Rolipram
(n = 4)
286 ± 7 0.17 ± 0.03 164 ± 14** 196 ± 10**
GF10903X (1 μM) + Rolipram
(n = 4)
282 ± 8 0.27 ± 0.03 152 ± 18** 200 ± 11**
FMLP (1 μM) Control
(n = 6)
285 ± 7 0.1 ± 0.01 94 ± 3 142 ± 9
GF10903X (1 μM)
(n = 11)
278 ± 4 0.53 ± 0.05* 54 ± 3* 107 ± 5*
  1. The results are expressed as the mean percentage of control ± S.E.M. * P < 0.05 for comparison with the untreated control system and **P < 0.05 for comparison with rolipram-treated cells. Basal Ca2+ concentrations were 80 ± 8 nM.