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Table 1 Effects of staurosporine and GF10903X, in the presence or absence of rolipram, on cytosolic Ca2+ concentrations of PAF- activated neutrophils, as well as the effects of GF10903X on cytosolic Ca2+ concentrations in FMLP-stimulated cells.

From: Protein kinase C promotes restoration of calcium homeostasis to platelet activating factor-stimulated human neutrophils by inhibition of phospholipase C

System

Peak

(nM)

Plateau

(min)

Magnitude of decrement (nM) from peak measured at:

   

1 min

2 min

PAF (20 nM) Control

(n = 6)

278 ± 16

0.4 ± 0.05

108 ± 10

168 ± 12

Staurosporine (400 nM)

(n = 4)

290 ± 8

1.0 ± 0.05*

52 ± 4*

160 ± 15

GF10903X (0.5 μM)

(n = 7)

258 ± 4

1.0 ± 0.04*

32 ± 2*

124 ± 5*

GF10903X (1 μM)

(n = 7)

266 ± 10

1.1 ± 0.02*

24 ± 3*

104 ± 4*

Rolipram (2 μM)

(n = 4)

274 ± 4

0.13 ± 0.03

188 ± 4

216 ± 4

Staurosporine + Rolipram

(n = 4)

286 ± 6

0.2 ± 0.06

144 ± 8**

200 ± 8

GF10903X (0.5 μM) + Rolipram

(n = 4)

286 ± 7

0.17 ± 0.03

164 ± 14**

196 ± 10**

GF10903X (1 μM) + Rolipram

(n = 4)

282 ± 8

0.27 ± 0.03

152 ± 18**

200 ± 11**

FMLP (1 μM) Control

(n = 6)

285 ± 7

0.1 ± 0.01

94 ± 3

142 ± 9

GF10903X (1 μM)

(n = 11)

278 ± 4

0.53 ± 0.05*

54 ± 3*

107 ± 5*

  1. The results are expressed as the mean percentage of control ± S.E.M. * P < 0.05 for comparison with the untreated control system and **P < 0.05 for comparison with rolipram-treated cells. Basal Ca2+ concentrations were 80 ± 8 nM.