Figure 4

Hcy-induced leukocyte cell adhesion to mesangial cells is abrogated by p38MAPK and PI-3 Kinase inhibitors (A) and by anti-MIP2 antibody (B). MC were incubated (24 hours/37°C) in presence of Hcy (50 μM) with or without inhibitors SB203580 (p38MAPK inhibitor; 10 μM) or LY294002 (PI3 Kinase inhibitor; 10 μM) or in the presence of pAb MIP-2 (5 μg/ml) B. L-Cys (100 μM) was used as a control. Cell adhesion assay was performed as described in method. The data represent mean ± SEM from three separate experiments; *p < 0.05; ^#p < 0.02.