IL-8 mRNA expression induced by IL-8 in human adipocytes. Human adipocytes were stimulated with IL-8 (50 nM or 100 nM) for 18 h (A). The IL-8 mRNA expression was analyzed by Northern blot. For the experiments with inhibitors (B), 0.1% DMSO was added as vehicle control, and PD98059 (50 μM) or SB203580 (50 μM) was added 2 h before stimulation with IL-8 (100 nM). The intensity of the IL-8 mRNA band was corrected with that of the 18s rRNA band. The data are representative of three different experiments (Means ± SD). * p < 0.01 vs. control # p < 0.05 vs. IL-8 50 nM.