Figure 4
From: Modulation of expression and cellular distribution of p21 by macrophage migration inhibitory factor
Decreased nuclear p21 in MIF-/- MDF compared with wt MDF. Immunofluorescent staining of p21 showing prominent nuclear localisation of p21 in wt MDF (a), in contrast to p21 expression in MIF-/- MDF showing diffuse staining in both the cytoplasm and nucleus (b). Reduced nuclear p21 protein expression was confirmed by flow cytometry (*P < 0.05) (c). In each group results are representative of 4 independent experiments.