MIF-/- cells exhibit reduced proliferative responses and increased apoptosis. a. Quantification of basal MIF-/- and wt MDF proliferation by 3H-thymidine incorporation. Significantly lower levels of thymidine incorporation were observed in MIF-/- MDF compared wt MDF, (N = 5, * P < 0.05). b. Proliferation of MIF-/- and wt MDF further quantified by haemocytometric cell count over 72 hours (N = 4, * P < 0.05). c. Apoptosis of MDF in response to cellular stress induced by 0.5 mM SNP was assessed by flow cytometric analysis of annexin V-FITC and PI. Percentage apoptosis represents all non-viable cells, (N = 4, * P < 0.05).