Effect of zVF on COX-2 mRNA. KD and Control (Con) shRNA MAEC were treated with vehicle (DMSO) or 25 μM zVF for 1 h, and then with medium or LPS/IFN for 1 h (A) or 24 h (B). mRNA for COX-2 and β-actin were assessed as in figure 3. Representative agarose electrophoresis of PCR products are shown. Replicate COX-2 or β-actin products in a single ethidium bromide-stained gel were imaged for analysis. Bars represent the mean + SE of ratio of COX-2/β-actin signal intensity + SE of 3 independent cultures. *: p < 0.05 for comparison between LPS/IFN- and medium-treated cells. +: p < 0.05 for comparison between KD and Control cells treated with LPS/IFN and zVF.