Figure 9

The effects of TMP on the growth and viability of RAW 264.7 cells. TMP was added to RAW 264.7 cells in vitro and their growth monitored for 24 h using the CyQuant proliferation assay (A). The induction of apoptosis in RAW 264.7 cells following a 24 h exposure to TMP was measured using an assay for active caspase 3 (Cayman) (B). In panels A and B, M and D indicate media and DMSO controls. In panel B, + and – indicate the addition of positive and negative controls supplied by the manufacturer. In C, cells were plated in 24 well plates, allowed to adhere 24 h, then washed and incubated with either fresh medium or medium containing 25 μM TMP. After 24 h, the medium containing TMP was removed from one set of wells, replaced with fresh medium without TMP, and the cells were incubated for an additional 48 h. Cell counts were determined by hemocytometer. Values shown are means +/- SEM and all points were performed in triplicate.