The effects of TMP on cytokine, chemokine and inflammatory lipid production by peritoneal macrophages. Macrophages were collected from C57BL6/J mice and left either untreated (Media), or treated overnight with 1 μg/ml LPS and/or 25 μM TMP. Levels of PGE2 (A), TNF-α (B), MCP-1 (C) and IL-12/23 p40 (D) in cell culture supernatants were subsequently determined by ELISA. Panels show means ± SEM from 2–3 experiments for each mediator. Asterisks indicate significant differences between treatments with LPS and LPS/TMP (p < 0.05, Student's t-test).