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Figure 1 | Journal of Inflammation

Figure 1

From: Terameprocol, a methylated derivative of nordihydroguaiaretic acid, inhibits production of prostaglandins and several key inflammatory cytokines and chemokines

Figure 1

Inhibition of prostaglandin production by TMP. RAW 264.7 cells were incubated with LPS and/or TMP for the indicated times and then PGE2 concentrations in culture supernatants were determined by ELISA (A). RAW 264.7 cells were incubated with LPS in the presence of increasing concentrations of TMP and PGE2 concentrations were determined by ELISA (B). RAW 264.7 cells were left untreated (Media) or treated with LPS and/or TMP and the concentrations in culture supernatants of PGF (C) and 6-keto-PGF (D) were determined by ELISA. RAW 264.7 cells were left untreated (Media) or treated with PMA (10 ng/ml) (E) or influenza A virus PR/8/34 (5 pfu/cell) (F) in the presence or absence of TMP and PGE2 concentrations in culture supernatants were determined by ELISA. Unless otherwise indicated concentrations of LPS and TMP were 1 μg/ml and 25 μM, respectively, and the incubation time was 16 h. Panels A and B show representative experiments while the other panels show the mean ± SEM from 3 experiments. All samples were assayed in duplicate and error bars are less than symbol size where not shown. TMP was added simultaneously in experiments with LPS or PMA while TMP was added 30 m after infection with influenza A. In panels C-F, asterisks indicate significant differences between treatments with inducing agents alone and inducing agents with TMP (p < 0.05, Student's t-test).

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