WFA pre-treatment inhibits IL-8 secretion from KKLEB cells stimulated with PAF. Cells were plated and treated as described in the "Findings" section. Treatment groups were as follows: Cells with media only (Untreated), cells treated with media containing 10% LB Broth (LB), cells treated with 10% PAF (PAF), cells pre-treated with various concentrations of WFA followed by stimulation with 10% PAF (WFA, 0.3, 1 and 3 μM). After 24 hr of incubation, media from each sample was collected and analyzed for IL-8 secretion using ELISA. Concentration of IL-8 was calculated by fitting the optical density of each sample to a standard curve prepared with recombinant IL-8 using linear regression. Data are reported as mean IL-8 secretion + standard deviation averaged from 3 samples per group. Withaferin pre-treated samples were compared to PAF-treated samples using Student's t-test. Statistical significance was noted at 3 μM as indicated.