Figure 2

WFA pre-treatment inhibits NFκB reporter activity in transiently transfected KKLEB cells stimulated with PAF. Cells were plated and treated as described in the "Findings" section. Treatment groups were as follows: Cells with media only (Untreated), cells treated with media containing 10% LB Broth (LB), cells treated with 10% PAF (PAF), cells pre-treated with various concentrations of WFA followed by stimulation with 10% PAF (WFA, 0.3, 1 and 3 μM). Data from luciferase reporter assays are reported as averaged arbitrary mean luminescence + standard deviation from 3 samples per group. Withaferin pre-treated samples were compared to PAF-treated samples using Student's t-test. Statistical significance was noted at 3 μM concentrations in WFA pre-treated cells compared to PAF only cells as indicated.