cGMP signalling inhibits apoptosis. MDMϕ were subjected to 24 h pre-treatment as shown below each column. A – The pre-treatments had no effect on cell viability after 24 h compared to vehicle (1% DMSO) treated cells (first column). B – The groups indicated were then treated for a further 24 h with GEA-3162 (100 μM). Pre-treatment with 8-bromo-cGMP, 300 μM DETA/NO, and 10 μM DETA/NO in combination with BAY 41–2272 or IBMX, all significantly attenuated GEA-3162-induced apoptosis. ODQ prevented the attenuation of apoptosis induced by 300 μM DETA/NO. **P < 0.01 significantly different from vehicle (1% DMSO) treated control cells (first column). #P < 0.01 significantly different from GEA-3162 treated cells (second column; one-way ANOVA followed by post hoc Dunnet's test; n = 3 – 9).