Inhibition of zymosan-induced kidney dysfunction by tyrphostin AG-490

Journal of Inflammation

Table 2 Effect of AG-490 on the number of IL-10-producing cells in peritoneum

Cell populations^a	Treatment^b	IL-10 producing cells (spots/1.0 × 10⁵cells)^c
		unstimulated	ZY-stimulated
Non-adherent peritoneal cells	Control	N.D.	N.D.
	ZY	4.0 ± 1.0	6.0 ± 2.8
	ZY + AG-490	9.0 ± 3.1*	10.0 ± 5.5
Adherent peritoneal cells	Control	N.D.	N.D.
	ZY	12.7 ± 6.8	27.0 ± 8.9
	ZY + AG-490	20.5 ± 4.5**	27.2 ± 7.2

^aPeritoneal cells were isolated 4 h after shock induction and were separated in non-adherent and adherent cell populations.
^bELISPOT assay was used to determine the number of IL-10-producing cells in peritoneal lavage from healthy mice (Control), untreated (ZY) or treated with AG-490 (ZY+AG-490) mice with shock. N.D denotes not detectable.
^cCell populations were cultured for 18 h in the absence (unstimulated) or the presence (ZY-stimulated) of zymosan.
Data are expressed as mean ± SD (n = 5/group) and were compared by Student's t test.
**p < 0.01 and *p < 0.05 versus zymosan-injected group.

ISSN: 1476-9255