Skip to main content
Figure 6 | Journal of Inflammation

Figure 6

From: Cigarette smoke regulates the expression of TLR4 and IL-8 production by human macrophages

Figure 6

CSM regulates phosphorylation of IRAK and degradation of IκB-α by MDMs and phosphorylation of IRAK in HEK cells. MDMs (3 × 106 cells) were pretreated with NAC (10 mM) for 30 min and then stimulated with CSM (0.06 OD) and LPS (100 ng/ml) for 30 min as described at material and methods section. The expression of phospho IRAK (A) and IκB-α degradation (B) were determined by whole lysates of cells by Western blot analysis. Representative results of three independent experiments and β-actin (C) served as loading controls from cytoplasm. D) TLR4 stably transfected HEK293 cells or 293-LacZ HEK-293 cells (3 × 106 cells) were stimulated with CSM for 30 min as described at material and methods section. The expression of phospho IRAK were determined by whole lysates of cells by Western blot analysis. Representative results of three independent experiments and β-actin served as loading controls from cytoplasm.

Back to article page