Figure 2

Modulation of TLR4 expression by CSM. TLR4 stably transfected HEK293 cell (A) or MDMs (B) were treated with CSM (0.03 and 0.06 OD) for 3 h and then incubated with PE conjugated anti-TLR4 or isotype control antibody as described in materials and methods. FACS analysis of a representative of at least 3 experiments showing the mean fluorescence intensity (MFI) difference of each group. Values are expressed as mean +/- S.E.M (n = 3). *p = 0.05,***p = 0.001 significantly different compared to control. C) MDMs were stimulated with CSM (0.06 OD) or LPS (100 ng/ml) for 3 h and then intracellular levels of TLR4 were measured as described in material and methods. Values are expressed as mean +/- S.E.M (n = 3). *p = 0.05, significantly different compared to control.