Figure 3

Effect of Plasma samples obtained before and 2 h after oral ingestion of PFE on IL-1β-induced NO production in rabbit chondrocytes. Confluent chondrocytes were serum starved and then treated with 200 μl of control or experimental plasma samples for 1 hr, stimulated with human IL-1β for 24 hrs. At the end of incubation, 100 μl of the medium was removed for measuring nitrite production by Griess reaction. Control values were obtained in the absence of plasma or IL-1β. Data were derived from two independent experiments, each run in triplicate, and expressed as Mean ± SE. Values without a common letter differ (P < 0.05 a vs b; P < 0.005, a vs c; b vs c).