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Figure 5 | Journal of Inflammation

Figure 5

From: IFN-gamma regulation of ICAM-1 receptors in bronchial epithelial cells: soluble ICAM–1 release inhibits human rhinovirus infection

Figure 5

Effect of IFN-γ cell preconditioning (1 ng/ml for 24 h) singly and with HRV-14 or HRV-1b infection (TCID 50 102.5 for 90 mins) on sICAM-1 protein release (A) and sICAM-1 gene expression (B) in NHBE cells over the study period. sICAM-1 in cell culture supernatants was assayed using ELISA. Data are mean ± S.E. of three separate experiments (Fig 5A,*p < 0.05, IFN-γ treated HRV-14 infected cells compared to IFN-γ treated uninfected cells and IFN-γ treated HRV-1b infected cells, **p = 0.02, IFN-γ treated infected cells compared to IFN-γ treated uninfected cells and IFN-γ treated HRV-1b infected cells). Gene expression of sICAM-1 was semi-quantified using RT-PCR and densitometry and expressed as a ratio to the housekeeping gene, G3PDH. Data are mean ± S.E. of three separate experiments

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