Figure 1
Effect of IFN-γ cell preconditioning (1 ng/ml for 24 h) singly and with HRV-14 or HRV-1b infection (TCID 50 102.5 for 90 mins) on mICAM-1 protein expression (A) and mICAM-1 gene expression (B) in NHBE cells over the study period (0–96 h; 0 h representing the point immediately after 90 min inoculation period). mICAM-1 protein expression was measured using immunofluorescence. Data are mean ± S.E. of three separate experiments (Fig. 1A, *p < 0.05, IFN-γ treated uninfected and infected cells compared to control untreated and uninfected cells, **p = 0.02, IFN-γ treated HRV-14 infected cells compared to IFN-γ treated uninfected cells and IFN-γ treated HRV-1b infected cells). Gene expression of mICAM-1 was semi-quantified using RT-PCR and densitometry and expressed as a ratio to the housekeeping gene, G3PDH. Data are mean ± S.E. of three separate experiments (Fig. 1B, *p < 0.05, IFN-γ treated infected cells compared to IFN-γ treated uninfected cells and IFN-γ treated HRV-1b infected cells).