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Figure 2 | Journal of Inflammation

Figure 2

From: JNK pathway is involved in the inhibition of inflammatory target gene expression and NF-kappaB activation by melittin

Figure 2

JNK inhibitor suppressed the inhibitory effect of melittin and bee venom on the NF-κB DNA binding activity induced by LPS or SNP. Raw 264.7 macrophages (A) and synoviocytes (B) were pretreated with 10, 20, and 50 μM SP600125 1 h prior to the treatment with melittin or bee venom with or without LPS or SNP for 2 h. The DNA binding activation of NF-κB was investigated using EMSA. Nuclear extracts from Raw 264.7 cells or synoviocytes treated for 1 hr were incubated with 32P-end-labeled oligonucleotide containing the κB sequence. Each panel is representative of three similar experiments with duplicates.

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