Effect of melittin and bee venom on LPS and SNP-induced phosphorylation of MAPKs. A, Raw 264.7 macrophages were treated with 5 or 10 μg/ml melittin or 0.5–5 μg/ml bee venom in the presence of LPS (1 μg/ml) or SNP (200 μM) at 37°C for 24 hr. B, Synoviocytes were treated with 5 or 10 μg/ml melittin or 0.5–5 μg/ml bee venom in the presence of 1 μg/ml LPS or 200 μM SNP at 37°C for 24 hr. Equal amounts of total proteins (80 μg/lane) were subjected to 10% SDS ± PAGE, and the expression of p-ERK/ERK, p-JNK/JNK, or p-p38/p38 were detected by western blotting using specific antibodies. Each panel representative of three independent experiments. Quantification of band intensities from three independent experimental results was determined by a densitometry (Imaging System). Data was described as means ± S.E. from three experiments performed in triplicate for p-ERK/ERK, p-JNK/JNK, or p-p38/p38. *p < 0.05 indicate statistically significant differences from the LPS or SNP-treated group.