Colonic TNF levels and histologic scores in control and LMP-420-treated IL-10-/- mice with chronic colitis. A, C. Levels of TNF in colonic tissues of IL-10-/- mice with chronic colitis following 16 days of treatment with i.p. (panel A) or oral (panel C) LMP-420 at the indicated doses. LMP-420 doses of 5 mg/kg/day i.p. and 41 mg/kg/day oral significantly decreased total colon tissue TNF content by 44% (p = 0.03) and 39% (p = 0.0003), respectively. B, D. Histologic scoring of colon tissues from the same mice showed a trend toward decreased histologic evidence of inflammation in mice treated with 41 mg LMP-420/kg/day orally (panel D), however this change approached (p = 0.06) but did not reach statistical significance. No decreases in histologic scores were seen in mice treated with i.p. LMP-420 (panel B), despite the decreased colonic TNF levels observed in panel A.