Skip to main content
Figure 2 | Journal of Inflammation

Figure 2

From: Cellular and molecular mechanisms of cigarette smoke-induced lung damage and prevention by vitamin C

Figure 2

A, Detection of DNA strand breaks by TUNEL assay in lung cells of vitamin C-restricted (1 mg vitamin C/animal/day) or vitamin C-supplemented (15 mg/animal/day) guinea pigs exposed to air CS (as described under Methods). Following CS exposure, the guinea pigs were sacrificed after 1, 3, 7, 14 and 21 days. Lower Panel: the lung sections were stained with fluorescein labeled dUTP according to the protocols discussed under Methods. Green fluorescence indicates TUNEL positive cells. Upper Panel: Lung sections corresponding to the upper panel were stained with DAPI to identify the cell nuclei. B, Quantitative evaluation of TUNEL positive cells in lungs of guinea pigs exposed to air or CS. Eight images were analyzed in 4 lung sections (2 images/section/animal) from each group, respectively; the bars over the respective columns represent means ± SD; * indicates p < 0.05 and ** indicates p < 0.001 with respect to sham controls. C, Colocalization of TUNEL and DAPI staining in the nuclei of lungs of guinea pig fed 1 mg vitamin C/day and exposed to CS for 21 days; original magnification × 63; image captured by confocal microscopy (Zeiss LSM 510 META).

Back to article page