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Figure 5 | Journal of Inflammation

Figure 5

From: Anti-oxidant inhibition of hyaluronan fragment-induced inflammatory gene expression

Figure 5

NAC and DMSO inhibit HA fragment-induced NF-κB, but not AP-1 activation. MH-S cells were transiently transfected with an NF-κB driven luciferase reporter construct for 18 h prior to stimulation with HA fragments (250 μg/ml) +/- 10 nM NAC (A) or DMSO 1% (B) for an additional 18 h. Cell lysates were harvested and assayed for luciferase expression and results are displayed as fold induction of luciferase activity. NCI H292 cells were transfected with NF-κB (C) or AP-1 (D) luciferase reporter constructs for 18 h prior to stimulation with HA fragments (250 μg/ml) +/- 10 nM NAC for an additional 18 h. Promoter activity was assayed by luciferase activity and results are displayed as fold induction of luciferase activity. These data represent the average of 6 identical, independent experiments.

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