Proteinase-activated receptor-2: two potential inflammatory mediators of the gastrointestinal tract in Atlantic salmon

Table 2 PCR primers used in cloning and real-time RT-PCR analysis

Gene name	Accession number	Primer name	PCR primer sequence	PCR annealing temperature (C°)	PCR product size (bp)
PAR-2	-	PAR-2F	ATCTACATGGCCAACCTGGC	58	149
PAR-2	-	PAR-2R_Deg	CAGTACAYGTTCCCGTAGAAGAA
PAR-2a	-	PAR-2a_RACE_F1	GTCCGACCTGCTCTTTGTCATCTGGA	68	1316*
PAR-2a	-	PAR-2a_RACE_R1	TGGACTCCCCTGAAGATTGCCTACCAC		739*
PAR-2b	-	PAR-2b_RACE_F1	CTGGACACCTCTGAAGATCGCCTACCAC		1655*
PAR-2b	-	PAR-2b_RACE_R1	GCCCACCAGGACTTTACACAGCCT		687*
PAR-2a	-	PAR-2a_RT_F1	GCGCTACTGTGCCATCGTCAA	60	104
PAR-2a	-	PAR-2a_RT_R1	TGGTCATCAGCCAGACCCCCA
PAR-2b	-	PAR-2b_RT_F1	ACGCTACTGGGGTGTGGCCCA		104
PAR-2b	-	PAR-2b_RT_R1	TGGTGGTGAGCCAGATGAAGG
ELF-1 α	AF321836	SS-EF1-alpha F1	GTGCTGTGCTTATCGTTGCT	60	148
ELF-1 α	AF321836	SS-EF1-alpha R1	GGCTCTGTGGAGTCCATCTT
β-actin	AF012125	SS beta-aktin F1	CAAAGCCAACAGGGAGAAGATGA	58	133
β-actin	AF012126	SS beta-aktin R1	ACCGGAGTCCATGACGATAC
GAPDH	BU693999	GAPDH F1	AAGTGAAGCAGGAGGGTGGAA	60	96
GAPDH	BU693999	GAPDH R1	CAGCCTCACCCCATTTGATG
18S rRNA	AJ427629	SS-18SrRNA F1	TACAGTGAAACTGCGAATGG	60	153
18S rRNA	AJ427629	SS-18SrRNA R1	GCATGGGTTTTGGGTCTG

PAR-2: Proteinase-activated receptor-2, ELF-1 α: Elongation factor 1 alpha, GAPDH: Glyceraldehyde-3-phosphate dehydrogenase. * PCR product size after RACE amplification