Plasma Treatment | Vmax (nM/min/ml) | Km (μM) | R2 | Ki ± s.e.m. (μM) |
|---|
Vehicle
| 1296 | 33.24 | 0.9938 | Â |
Palmitoyl
| 684.7 | 16.12 | 0.9595 | 25.9 ± 4.0 |
Vehicle
| 1395 | 27.24 | 0.9413 | Â |
Stearoyl
| 669.2 | 11.26 | 0.928 | 34.2 ± 17.4 |
- Data from enzyme reactions of plasma samples containing lysoPC (20 μM) or vehicle. The reactions for each lysoPC–containing sample were run side by side with vehicle-containing samples and they were drawn from the same pool of plasma. In each case the lysoPC reduced both Km and Vmax showing the inhibition was uncompetitive. Average Ki values with s.e.m. were calculated as in previous studies from all reactions with substrates above 15 μM [23]. R2 = fit to Michaelis-Menten function.
