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Figure 1 | Journal of Inflammation

Figure 1

From: 15-deoxy-delta12,14-prostaglandin J2 attenuates endothelial-monocyte interaction: implication for inflammatory diseases

Figure 1

5d-PGJ2 inhibits monocyte adhesion to endothelial cells. bEND.3 cells were incubated with different concentrations of 15d-PGJ2 (1–20 μM) (A) or mentioned prostaglandins (5 μM), arachidonic acid (5 μM), Leukotriene 4 (LTB 4, 5 μM) and Thromboxanes 4 (TXB 4, 5 μM) (C) for 30 min followed by TNFα (50 ng/ml) stimulation for 6 h. Fluorescently labeled monocytes were allowed to interact with activated bEND.3 cells. Adhered monocytes were counted as mentioned in 'Material and Methods'. Data calculated as mean ± SD of 21 fields from 3 different experiments. *** p < 0.001 compared to untreated control cells and !!! p < 0.001 compared to TNFα treated cells. (B) is the pictorial representation of adhesion under TNFα (50 ng/ml) and 15d-PGJ2 (10 μM) treatment.

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