Figure 4
From: Black tea prevents cigarette smoke-induced apoptosis and lung damage
Detection of DNA strand breaks in lung cells of guinea pigs exposed to air or CS in the presence or absence of BT by TUNEL assay. The guinea pigs were exposed to air or CS (as described under Materials and Methods) and sacrificed after 7 days of exposure. Lower Panel: the lung sections were stained with fluorescein labeled dUTP according to the protocols discussed under 'Materials and Methods'. A, guinea pigs exposed to air and given water as a drink; B, guinea pigs exposed to CS and given water as a drink; C, guinea pigs exposed to air and given BT as the drink; D, guinea pigs exposed to CS and given BT as the drink. Upper Panel: Lung sections corresponding to the upper panel were counterstained with DAPI to identify the cell nuclei.