Figure 5

Effect of bisindolylemaleimide (Bis) on LPS stimulated NF-κB activation in RAW 264.7 cells. RAW cells were stimulated with LPS (1 μg/ml) in the presence or absence of Bis (20 μM) for 3 hr. Cells were harvested and nuclear NF-κB-p65 levels were assessed by Western blotting. (a) Representative Western blot of p65 expression. Equal amounts of nuclear proteins were loaded onto each lane. (b) Graphical representation of % increase in p65 nuclear localisation is shown in (a) above. Data is presented as mean ± SEM, n = 3 independent measurements.