Figure 4

Effect of PKC inhibitors on LPS induced NO production in RAW 264.7 cells. Cells were treated with vehicle (control) or LPS (1 μg/ml) in the presence or absence of PKC inhibitors Go 6978 (Go, 10 μM) or bisindolylemaleimide (Bis, 20 μM) for 24 hours. The culture medium was then harvested and assayed for nitrite content by Greiss assay. The data show that only Bis was able to inhibit LPS-stimulated RAW cell NO production. Results are expressed mean ± SEM, ***p < 0.001, n = 9.