Figure 5
From: Sensitivity of mice to lipopolysaccharide is increased by a high saturated fat and cholesterol diet
The effect of diet and LPS on hepatic iNOS expression. iNOS mRNA (A) was assessed as in figure 4. Values are expressed mean ± SE of 5 mice are the fold increases in iNOS/β-actin mRNA signal ratio relative CD mice treated with 0 mg LPS/ml, represented by 1 on the y-axis. The level of iNOS protein (B) was measured by western blotting 4 and 12 h after treatment (representative images and image analysis). Values are the mean + SE of total integrated signal intensity obtained from densitometry for 5 mice in each group. *: p < 0.05 for comparison with 0 mg LPS/kg. +: p < 0.05 for comparison with CD treated with the same dose of LPS.