Figure 4
From: Sensitivity of mice to lipopolysaccharide is increased by a high saturated fat and cholesterol diet
The effect of diet and LPS on hepatic VCAM-1 expression. mRNA levels (A) were measured with RT-PCR. Densitometric intensity of images of ethidium bromide-stained agarose gels were normalized to β-actin RT-PCR signal. Induction of VCAM-1 RT-PCR signal ratios (n = 5 in each group) are expressed as fold increase compared to vehicle-treated CD mice, represented by 1 on the y-axis. Values are expressed mean ± SE of 5 mice are the fold increases in VCAM-1/β-actin mRNA signal ratio relative CD mice treated with 0 mg LPS/ml (represented by 1 on the y-axis). The level of VCAM-1 protein (B) was measured by western blotting 4 and 12 h after treatment (representative images and image analysis). Values are the mean + SE of total integrated signal intensity obtained from densitometry for 5 mice in each group. *: p < 0.05 for comparison with 0 mg LPS/kg. +: p < 0.05 for comparison with CD treated with the same dose of LPS.