Figure 5
From: Alveolar macrophages lack CCR2 expression and do not migrate to CCL2
Peripheral blood monocytes and alveolar macrophages differentially express the CCL3 receptors CCR1 and CCR5. Freshly isolated monocytes and alveolar macrophages (5 × 105/condition) were assayed for surface expression of CCR1 (left panels) and CCR5 (right panels) using flow cytometry. (a) Monocytes expressed CCR1 but (d) not CCR5. For monocytes, the average fold-increase in median fluorescence over IgG when staining for (c) CCR1 was 2.37 ± 0.53 (*p < 0.05 versus IgG controls), and when staining for (f) CCR5 was 1.07 ± 0.04 (p > 0.05 versus IgG controls). Alveolar macrophages expressed (d) CCR5, but not (b) CCR1. For alveolar macrophages, the average fold-increase in mean fluorescence over IgG when staining for (c) CCR1 was 1.04 ± 0.04 (p > 0.05 versus IgG controls), and when staining for (f) CCR5 was 1.45 ± 0.17 (*p < 0.05 versus IgG controls). IgG isotype controls are shown (dashed lines). Data are representative of three independent experiments and graphs represent mean ± SEM.