Figure 2
From: Alveolar macrophages lack CCR2 expression and do not migrate to CCL2
CCR2 surface protein is expressed at lower levels in alveolar macrophages compared to freshly isolated blood monocytes. (a) Monocytes (5 × 105 per condition) and (b) alveolar macrophages (5 × 105/condition) were isolated from the same donor and subjected to flow cytometric staining for CCR2 (solid line). (c) The average fold increase in CCR2 median fluorescence over isogenic IgG was 2.64 ± 0.48 for monocytes (*p < 0.05) and 1.12 ± 0.10 for alveolar macrophages (p > 0.05) for three independent experiments. IgG isotype controls are represented by dashed lines.