Characterisation of TGFβ1 transgenic mice. A. Expression of the transgene in wild type and both line 18 and line 25 transgenic mice was assessed by PCR using TGFβ1 sequence specific primers. This figure is a representative agarose gel post amplification indicating expression of the TGFβ1 transgene in both line 18 and line 25 Tr+ transgenic mice (Lanes 1 and 2). Lanes 3 and 4 show absence of transgene in wild type mice. Figure B and C show PAIL lumineriferase assay results. To determine the effect of the transgene on circulating TGFβ1, both total (B) and active (C) TGFβ1 concentrations in sera was determined.