Soy isoflavones avert chronic inflammation-induced bone loss and vascular disease

Journal of Inflammation

Table 5 TNF-α expression in bone

Score		Cells Expressing TNF-α		Intensity of TNF-α Expression		Overall Score* (Cells Expressing × Intensity of Expression)
		%	$\bar{X}$	%	$\bar{X}$
0 IF Placebo	1	0	2.0 ± 0.3	0	2.0 ± 0.0	5.0 ± 0.6^b
	2	50		100
	3	50		0
	4	0		0
126 IF Placebo	1	33.3	1.7 ± 0.3	100	1.0 ± 0.0	1.7 ± 0.3^c
	2	66.6		0
	3	0		0
	4	0		0
504 IF Placebo	1	33.3	1.7 ± 0.3	100	1.0 ± 0.0	1.7 ± 0.3^c
	2	66.6		0
	3	0		0
	4	0		0
0 IF LPS	1	0	4.0 ± 0.0	0	4.0 ± 0.0	16.0 ± 0.0^a
	2	0		0
	3	0		0
	4	100		100
126 IF LPS	1	33.3	1.3 ± 0.3	33.3	1.7 ± 0.3	2.0 ± 0.0^c
	2	66.6		66.6
	3	0		0
	4	0		0
504 IF LPS	1	100	1.0 ± 0.0	33.3	1.7 ± 0.3	1.7 ± 0.3^c
	2	0		66.6
	3	0		0
	4	0		0

Mice were fed soy isoflavones (IF; 0, 126 or 504 mg aglycone equivalents of IF/kg diet) for 14-days prior to and during a 30-day exposure to LPS (1.33 μg/d). TNF-α expression was determined immunhistochemically. Chi-square analysis was performed on the % of cells expressing TNF-α and the intensity of expression. The overall score was calculated. Results for the overall score are expressed as means ± standard error. Values for a given parameter that share the same superscript letter are not statistically different (P > 0.05) from each other. P-value for LPS*Diet on overall scores is < 0.0001.

ISSN: 1476-9255