Figure 1

Tumor-necrosis-α expression in proximal tibia metaphysis. Micrographs (20x) from immunohistochemical staining for TNF-α in the proximal tibia metaphysis of mice following the feeding of soy isoflavones (IF; 0, 126 or 504 mg aglycone equivalents of IF/kg diet) for 14-days prior to and during a 30-day exposure to LPS (1.33 μg/d). Tibial sections shown are from mice administered: 0 LPS (placebo pellets) with either (A) 0 IF, (B) low IF (126 mg IF/kg diet), or (C) high IF (504 mg IF/kg diet); or LPS (pellets releasing 1.33 μg/d) with either (D) 0 IF, (E) low IF (126 mg IF/kg diet), or (F) high IF (504 mg IF/kg diet). The representative sections demonstrate an increase in expression of TNF-α with LPS (arrow in D) and a down-regulation of expression with the low (E) and high (F) IF doses.