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Table 3 TAC of Mahonia crude extract, fraction Iand II and two representative alkaloids.

From: Free radical scavenging activity and lipoxygenase inhibition of Mahonia aquifolium extract and isoquinoline alkaloids

  TAC, μg Trolox equiv/g
Fraction I 209 833.3 ± 4880.0
Fraction II 39 969.7 ± 3007.3
Mahonia crude extract 71 406.8 ± 5822.6
Jatrorrhizine 116 519.5 ± 1601.4
Berberine 38 441.6 ± 3171.3
  1. A total 100 μl sample was mixed with a 2900 μl ethanol solution of DPPH (final concentration 60 μM), and the reaction continued for 5 hours. The final concentrations in the reaction mixture of the samples tested were as follows: 0.027 mg/ml for the fractions; 0.016 mg/ml for the standard compounds and 0.023 mg/ml for crude extract in water. Total antioxidant capacity (TAC) was calculated using standard curves for the reaction of Trolox with DPPH and the absorbance change at 515 nm after the reaction time, and the readings were then used to calculate TAC in the sample, expressed in μg Trolox equivalents/g. Each result is expressed as mean ± S.D. for three values.