Skip to main content

Table 1 Results of lipoxygenase inhibitory effects of crude Mahonia extract and two representative alkaloid fractions tested.

From: Free radical scavenging activity and lipoxygenase inhibition of Mahonia aquifolium extract and isoquinoline alkaloids

 

Final concentration (g/L)

Activity of lipoxygenase (kat)

% Inhibition

IC50 (g/L)

Crude Mahonia extract

0.50.10-3

7.40 ± 0.89

43.03 ± 0.54

0.76.10-3 ± 0.12.10-3

 

0.75.10-3

6.34 ± 7.63

51.19 ± 0.96*

 
 

1.25.10-3

5.2 8 ± 0.78

59.32 ± 1.39*

 
 

1.88.10-3

3.53 ± 0.34

72.80 ± 1.54*

 
 

2.50.10-3

2.92 ± 0.28

78.80 ± 1.72*

 

Control

-

12.99 ± 1.23

-

 

Fraction I

    
 

1.25.10-3

1.16 ± 0.11

16.54 ± 7.67

4.63.10-3 ± 1.03.10-3

 

2.50.10-3

0.94 ± 0.09

32.23 ± 2.63

 
 

3.75.10-3

0.71 ± 0.09

47.33 ± 2.24

 
 

5.10-3

0.63 ± 0.07

54.67 ± 1.64*

 
 

6.25.10-3

0.55 ± 0.06

60.43 ± 2.36*

 
 

7.50.10-3

0.34 ± 0.04

75.39 ± 2.10*

 

Control

-

1.39 ± 0.14

-

 

Fraction II

    
 

1.25.10-3

1.24 ± 0.12

10.93 ± 4.76

6.10.10-3 ± 0.98.10-3

 

2.50.10-3

1.11 ± 0.10

18.27 ± 6.23

 
 

3.75.10-3

0.95 ± 0.09

31.08 ± 2.25

 
 

5.10-3

0.87 ± 0.09

39.28 ± 2.47

 
 

6.25.10-3

0.64 ± 0.07

53.67 ± 2.33*

 
 

7.50.10-3

0.56 ± 0.06

60.86 ± 3.04*

 

Control

-

1.39 ± 0.24

-

 
  1. Lipoxygenase activity was determined as absorbance increase at λmax = 234 nm at 3 minutes of incubation with or without inhibitor tested. Values of hydroperoxide content and lipoxygenase activity were calculated from equation c = A.V/ε.l.v, where A is the value of absorbance increase, V is the volume of incubation mixture, ε is the extinction coefficient for linoleic acid (25.10-3 mol.l.cm-1), l is the length of the cuvette (1 cm) and v is the volume of enzyme (0.015 ml). Results are presented as percent of control ± SD, n = 3, * p < 0.05 vs. controls
Back to article page

Journal of Inflammation

ISSN: 1476-9255

Contact us