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Table 1 Results of lipoxygenase inhibitory effects of crude Mahonia extract and two representative alkaloid fractions tested.

From: Free radical scavenging activity and lipoxygenase inhibition of Mahonia aquifolium extract and isoquinoline alkaloids

  Final concentration (g/L) Activity of lipoxygenase (kat) % Inhibition IC50 (g/L)
Crude Mahonia extract 0.50.10-3 7.40 ± 0.89 43.03 ± 0.54 0.76.10-3 ± 0.12.10-3
  0.75.10-3 6.34 ± 7.63 51.19 ± 0.96*  
  1.25.10-3 5.2 8 ± 0.78 59.32 ± 1.39*  
  1.88.10-3 3.53 ± 0.34 72.80 ± 1.54*  
  2.50.10-3 2.92 ± 0.28 78.80 ± 1.72*  
Control - 12.99 ± 1.23 -  
Fraction I     
  1.25.10-3 1.16 ± 0.11 16.54 ± 7.67 4.63.10-3 ± 1.03.10-3
  2.50.10-3 0.94 ± 0.09 32.23 ± 2.63  
  3.75.10-3 0.71 ± 0.09 47.33 ± 2.24  
  5.10-3 0.63 ± 0.07 54.67 ± 1.64*  
  6.25.10-3 0.55 ± 0.06 60.43 ± 2.36*  
  7.50.10-3 0.34 ± 0.04 75.39 ± 2.10*  
Control - 1.39 ± 0.14 -  
Fraction II     
  1.25.10-3 1.24 ± 0.12 10.93 ± 4.76 6.10.10-3 ± 0.98.10-3
  2.50.10-3 1.11 ± 0.10 18.27 ± 6.23  
  3.75.10-3 0.95 ± 0.09 31.08 ± 2.25  
  5.10-3 0.87 ± 0.09 39.28 ± 2.47  
  6.25.10-3 0.64 ± 0.07 53.67 ± 2.33*  
  7.50.10-3 0.56 ± 0.06 60.86 ± 3.04*  
Control - 1.39 ± 0.24 -  
  1. Lipoxygenase activity was determined as absorbance increase at λmax = 234 nm at 3 minutes of incubation with or without inhibitor tested. Values of hydroperoxide content and lipoxygenase activity were calculated from equation c = A.V/ε.l.v, where A is the value of absorbance increase, V is the volume of incubation mixture, ε is the extinction coefficient for linoleic acid (25.10-3 mol.l.cm-1), l is the length of the cuvette (1 cm) and v is the volume of enzyme (0.015 ml). Results are presented as percent of control ± SD, n = 3, * p < 0.05 vs. controls