Figure 9
HPLC determination of ketamine concentration in plasma from rats receiving nebulized ketamine. The retention times of ketamine and phenacetin (internal calibrator) in the utilized system were ~5.87 and 2.58 min, respectively. (A) Chromatograms of blank plasma supplemented with 500 μg/L of a ketamine standard. (B) Representative chromatogram of plasma sample collected from a normal rat at 0 min after inhalation of 25 mg/ml ketamine. (C) Plasma concentration-time profiles of ketamine over a period of 60 min after the rats received nebulized ketamine at concentration of 12.5, 25 or 50 mg/ml respectively. The values represent the mean ± SEM (n = 6/group).