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Table 2 Ttp family and other selected mRNA levels in rat liver and muscle

From: Green tea increases anti-inflammatory tristetraprolin and decreases pro-inflammatory tumor necrosis factor mRNA levels in rats

Tissue mRNA Cycle of threshold (CT ± SD) Expression ratio (relative to Ttp) (Fold) Expression ratio (relative to liver) (Fold)
Liver Rpl32 18.35 ± 0.40 Internal control 1.00
  Ttp/Tis11/Zfp36 21.23 ± 0.43 1.00 1.00
  Zfp36l1/Tis11b 20.67 ± 0.54 1.48 1.00
  Zfp36l2/Tis11d 25.19 ± 0.36 0.06 1.00
  Zfp36l3 0.00 0.00  
  Tnf 39.12 ± 0.77   1.00
  Csf2/Gm-csf 0.00   
  Ptgs2/Cox2 33.09 ± 0.39   1.00
  Vegfa 20.36 ± 0.27   1.00
  Vegfb 25.69 ± 0.56   1.00
  Elavl1/Hua/Hur 22.46 ± 0.22   1.00
Muscle Rpl32 18.26 ± 0.26 Internal control 1.03
  Ttp/Tis11/Zfp36 23.81 ± 0.97 1.00 0.21
  Zfp36l1/Tis11b 24.32 ± 0.85 0.70 0.07
  Zfp36l2/Tis11d 26.51 ± 0.85 0.15 0.49
  Zfp36l3 0.00 0.00  
  Tnf 40.05 ± 0.55   0.59
  Csf2/Gm-csf 0.00   
  Ptgs2/Cox2 31.55 ± 0.83   2.42
  Vegfa 21.14 ± 0.35   0.59
  Vegfb 23.66 ± 0.29   4.27
  Elavl1/Hua/Hur 23.54 ± 0.18   0.42
  1. Twenty-five ng of RNA-derived cDNAs were used for the quantitation of mRNA levels using 40–60 cycles of real-time PCR program. The mean CT (cycle of threshold) values and standard deviations (n = 5–8) are shown in the table. The relative ratios of mRNA levels were calculated using the double delta CT method normalized with Rpl32 CT value as the internal control and Ttp CT value or liver CT value as the calibrator.