Green tea increases anti-inflammatory tristetraprolin and decreases pro-inflammatory tumor necrosis factor mRNA levels in rats

Table 2 Ttp family and other selected mRNA levels in rat liver and muscle

Tissue	mRNA	Cycle of threshold (C_T ± SD)	Expression ratio (relative to Ttp) (Fold)	Expression ratio (relative to liver) (Fold)
Liver	Rpl32	18.35 ± 0.40	Internal control	1.00
	Ttp/Tis11/Zfp36	21.23 ± 0.43	1.00	1.00
	Zfp36l1/Tis11b	20.67 ± 0.54	1.48	1.00
	Zfp36l2/Tis11d	25.19 ± 0.36	0.06	1.00
	Zfp36l3	0.00	0.00
	Tnf	39.12 ± 0.77		1.00
	Csf2/Gm-csf	0.00
	Ptgs2/Cox2	33.09 ± 0.39		1.00
	Vegfa	20.36 ± 0.27		1.00
	Vegfb	25.69 ± 0.56		1.00
	Elavl1/Hua/Hur	22.46 ± 0.22		1.00
Muscle	Rpl32	18.26 ± 0.26	Internal control	1.03
	Ttp/Tis11/Zfp36	23.81 ± 0.97	1.00	0.21
	Zfp36l1/Tis11b	24.32 ± 0.85	0.70	0.07
	Zfp36l2/Tis11d	26.51 ± 0.85	0.15	0.49
	Zfp36l3	0.00	0.00
	Tnf	40.05 ± 0.55		0.59
	Csf2/Gm-csf	0.00
	Ptgs2/Cox2	31.55 ± 0.83		2.42
	Vegfa	21.14 ± 0.35		0.59
	Vegfb	23.66 ± 0.29		4.27
	Elavl1/Hua/Hur	23.54 ± 0.18		0.42

Twenty-five ng of RNA-derived cDNAs were used for the quantitation of mRNA levels using 40–60 cycles of real-time PCR program. The mean C_T (cycle of threshold) values and standard deviations (n = 5–8) are shown in the table. The relative ratios of mRNA levels were calculated using the double delta C_T method normalized with Rpl32 C_T value as the internal control and Ttp C_T value or liver C_T value as the calibrator.

ISSN: 1476-9255