Figure 4
Effects of NF161 on PMN adhesion to HUVEC evoked by PAF, FMLP, IL-1β, TNF-α and PMA. HUVEC were pretreated with increasing concentrations (10-6–10-4M) of the tested compound for 10 min at 37°C and then challenged with PAF, FMLP (both at 10-7M) or PMA (10-8M) and PMNs for a further 10 min; IL-1β and TNF-α (both at 10 ng/ml) were incubated 1 h with the pretreated HUVEC and then challenged with PMNs for a further 10 min. Data are expressed as percentage inhibition versus control adhesion. Control adhesion was 51 ± 9 cells/microscope field (mean ± SEM, n = 26). PAF, FMLP, IL-1β, PMA and TNF-α stimulation vs control was, respectively: 305 ± 37%, 283 ± 28%, 270 ± 23%, 340 ± 46% and 304 ± 29%. Data are expressed as means ± SEM; n = 5. IC50 values were: 9.2 ± 0.7 × 10-6M, 3.7 ± 0.3 × 10-5M, 6.4 ± 0.4 × 10-5M and 3.1 ± 0.5 × 10-5M when the stimulus used was PAF, FMLP, IL-1β and TNF-α, respectively. IC50 values obtained with PAF were statistically different from all others (P < 0.01). Asterisks mark statistically significant inhibition of NF161 using PAF as stimulus versus all other stimuli (**P < 0.01).